This chapter also details cell preparation and production of the hybridization probes adapted to the comet-FISH technique. If you are planning on keeping more than one species of fish in your tank, you will need a separate tank. This will allow you to have plenty of room for all of the fish you want to keep in the tank. If possible, we recommend aiming for a 75-gallon tank. Go with something a bit larger if you can. The neutral version of the comet assay detects double-strand breaks, while the alkaline version detects both double- and single-strand breaks as well as abasic sites or sites of incomplete repair. The minimum tank size should be 40 or 50 gallons. Add these guys to your livestock water trough You should never need to feed them supplement foods if raised outdoors as they will feed on larvae, bugs and algae. are always happy to take pictures of specific fish in our tanks upon request. The Comet Goldfish, also called the Comet-tail Goldfish, are a ‘reddish orange’ colored goldfish that will be a delightful addition to your pond or water feature. A detailed description of the comet assay is given in Chapter 9, so readers who are not familiar with this technique can work directly with the protocol described here, without referring to additional protocols reported elsewhere. Most people have kept or at least heard of the Comet Goldfish with a flowing. Comet-FISH exists in two versions, based on the neutral and the alkaline comet assays. It is a combination of two established techniques, the comet assay (or single-cell gel electrophoresis, or the single-cell gel test), to separate highly fragmented from moderately or nonfragmented DNA and to measure it, and fluorescence in situ hybridization (FISH), to specifically label DNA sequences of interest. The comet-FISH technique described in this protocol is a tool to detect genome region-specific DNA damage and repair.
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